发明名称 |
SCREENING USING S. CEREVISIAE MANNOSYLTRANSFERASE ENCODING GENES |
摘要 |
The present invention relates to an antifungal in vitro and in vivo screening assays for identifying compounds which inhibit mannosyltransferases involved in protein O- and N-glycosylation. The antifungal screening assay for identifying a compound which inhibits mannosyltransferases involved in protein O- and N-glycosylation, comprises the steps of: a) subjecting proteins to a specific mannosyltransferase protein encoded by a Saccharomyces cerevisiae mannosyltransferase encoding gene, wherein said gene is selected from the group consisting of KRE2/MNT1, YUR1, KTR1, KTR2, KTR3, KTR4, KTR5, KTR6 and KTR7; b) subjecting step a) to a screened compound and determining the absence or presence of protein O- and N-glycosylation, wherein the absence of protein O- and N-glycosylation is indicative of an antifungal compound. There is also disclosed an in vitro method for the diagnosis of diseases caused by fungal infection in a patient.
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申请公布号 |
WO9947698(A1) |
申请公布日期 |
1999.09.23 |
申请号 |
WO1999CA00227 |
申请日期 |
1999.03.16 |
申请人 |
MCGILL UNIVERSITY;BUSSEY, HOWARD;LUSSIER, MARC;SDICU, ANNE-MARIE |
发明人 |
BUSSEY, HOWARD;LUSSIER, MARC;SDICU, ANNE-MARIE |
分类号 |
C12Q1/18;C12Q1/48;(IPC1-7):C12Q1/18;C12Q1/02 |
主分类号 |
C12Q1/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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