发明名称 |
Electrochemiluminescence assay, especially for biological substances, with long measurement period |
摘要 |
Detection of an analyte in a sample is performed using a label comprising a metal complex containing at least one charge carrier and/or at least one hydrophilic group. Detecting an analyte in a sample by electrochemiluminescence comprises using a label comprising a metal complex containing at least one charge carrier and/or at least one hydrophilic group and measuring the electrochemiluminescence for at least 0.5 seconds. An Independent claim is also included for detecting an analyte in a sample by electrochemiluminescence, comprising: (a) providing an electrochemiluminescence device comprising a measuring electrode; (b) contacting the electrode with a conditioning fluid containing an electrochemiluminescence co-substrate; (c) adjusting the conditions at the electrode so that activated co-substrate molecules are generated on and/or in the interfacial region of the electrode; (d) contacting the electrode with a sample containing an electrochemiluminescence co-substrate and a label comprising a metal complex containing at least one charge carrier and/or at least one hydrophilic group; (e) applying an electrochemiluminescence-inducing potential to the electrode and measuring the electrochemiluminescence; and (f) correlating the measured luminescence with the presence or amount of the analyte in the sample. |
申请公布号 |
DE19811582(A1) |
申请公布日期 |
1999.09.23 |
申请号 |
DE1998111582 |
申请日期 |
1998.03.17 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
PUNZMANN, GABRIELE;EGGER, MARTIN;JOSEL, HANS-PETER |
分类号 |
G01N21/76;G01N21/66;G01N27/416;G01N27/42;G01N33/58;(IPC1-7):G01N21/66;G01N33/52;G01N33/53 |
主分类号 |
G01N21/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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