Analyzing cell contents, especially nucleic acids, useful for medical diagnostics

摘要

A process for the analysis of cell contents, especially nucleic acids, uses a substrate specific for the cell component being analyzed bound to macroscopic particles, and ultrasound energy, to produce an analyte with is then subjected to PCR analysis. The new process comprises: (1) adding macroscopic particles comprising a cell content-specific substrate on their surface to a buffered cell solution; (2) lyzing the cells and isolating of the cell contents using ultrasound energy; (3) separation and washing of the macroscopic particle-bound cell contents from the buffer solution within the reaction vessel; and (4) drying of the complexes; and (5) amplification and detection of the cell contents using polymerase chain reaction (PCR). An Independent claim is also included for a device for carrying out the method described above.