Verfahren zur Synthese von stabiler, einzelsträndiger cDNS in Eukaryoten durch Transfektion mit einem bakteriellen Retron enthaltenden Vektor und der verwendete Vektor
摘要
A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by an RNA and a DNA portion. The group of genes (retrons) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. Utilities are disclosed. <IMAGE>
申请公布号
DE69228098(T2)
申请公布日期
1999.07.01
申请号
DE1992628098T
申请日期
1992.08.28
申请人
UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY, NEWARK, N.J., US