发明名称 |
HETEROZYGOTE SCREENING PROCESS |
摘要 |
In order to facilitate the screening of an organism, or a population of organisms, carrying heterozygous mutations for identifying the presence of a mutation in a gene of interest, a process is provided which utilises mismatch binding proteins, such as MutS. The process comprises the steps of: obtaining a nucleic acid sample from the organism; denaturing any double stranded nucleic acid present in the sample; allowing the nucleic acid to anneal; and removing homoduplexes from the annealed sample. A positive signal from a probe specific for the gene of interest indicates that the organism carries a heterozygous mutation in said gene.
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申请公布号 |
CA2311526(A1) |
申请公布日期 |
1999.05.27 |
申请号 |
CA19982311526 |
申请日期 |
1998.11.19 |
申请人 |
HEXAGEN TECHNOLOGY LIMITED |
发明人 |
FOSTER, JAMIE WILLIAM;SCHAFER, ALAN JOHN |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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