METHOD OF INCREASING EFFICIENCY OF DIRECTED EVOLUTION OF A GENE USING PHAGEMID

摘要

An improved method of mutating DNA is provided comprising mutating a gene bearing phagemid in a host and infecting the host cell with a suitable helper phage to initiate packaging of the DNA into a phagemid. The packaged phagemid is then used to infect a second host cell. The method facilitates more efficient screening for genetic diversity. Also, the gene of interest is mutated and separable from the host cell which undergoes the mutational event reducing false positive results or other artifacts of general mutation techniques.