Method for detecting molecules containing nucleotide mismatches and the location of these mismatches, and application to the detection of base substitutions or deletions in nucleotide sequences

摘要

PCT No. PCT/FR94/01068 Sec. 371 Date Jul. 15, 1996 Sec. 102(e) Date Jul. 15, 1996 PCT Filed Sep. 9, 1994 PCT Pub. No. WO95/07361 PCT Pub. Date Mar. 16, 1995Method for the detection and/or location of mutations or deletions in nucleotide sequences by creation of heteroduplexes between two types of double-stranded DNA able to form mismatches at the sites of said mutations or deletions. The heteroduplexes are detected as a result of the labeling of each type of strand by different fluorescent molecules or are screened by passage over a support which specifically retains them.