IMPROVEMENTS IN OR RELATING TO EXPRESSION OF NUCLEIC ACID SEQUENCES IN CEREBELLAR CELLS

摘要

Disclosed is a nucleic acid fragment which, when operably linked to a downstream nucleic acid sequence of interest, causes selective expression in cerebellar granule cells of the downstream sequence, the nucleic acid fragment comprising an isolated 5' portion of the gamma -aminobutyric acid type A (GABAA) receptor alpha 6 subunit gene, the isolated portion comprising: the first seven exons, and a 5' part of exon 8 of the alpha 6 subunit gene, the intervening introns, and at least 500 bp upstream of the transcription start site region. Also disclosed are constructs and host cells comprising the fragment, and methods of expressing nucleic acid sequences in cerebellar granule cells.