发明名称 CATEGORISING NUCLEIC ACID
摘要 <p>Provided is a method for characterising one or more nucleic acids, which method comprises immobilising double-stranded nucleic acids on a solid phase support, cleaving the immobilised nucleic acids with an endonuclease such that each cleaved nucleic acid has a double-stranded portion, denaturing the cleaved nucleic acids to form single-stranded cleaved nucleic acid, hybridising one or more oligonucleotide sequences to the resulting single-stranded cleaved nucleic acid, each oligonucleotide sequence comprising a pre-determined recognition sequence situated such that it recognises a sequence which was part of the double-stranded portion of the nucleic acid and a label specific to the recognition sequence, extending correctly hybridised oligonucleotide sequences along the single-stranded portion of the immobilised nucleic acid to form an extended strand, denaturing the extended strand from the immobilised strand and characterising the immobilised nucleic acid by identifying the size of the extended strand and the identity of the recognition sequence.</p>
申请公布号 WO1999002727(A1) 申请公布日期 1999.01.21
申请号 GB1998002045 申请日期 1998.07.13
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