发明名称 |
Lawn assay for compounds that affect enzyme activity or bind to target molecules |
摘要 |
A lawn assay is described for determining compounds that affect enzyme activity or that bind to target molecules. Compounds to be screened are cleaved, and diffused from solid supports into a colloidal matrix. Enzymatic catalysis or binding to target molecules by the compounds is carried out in the matrix. Active compounds are found by monitoring a photometrically detectable change in a substrate, coenzyme, or cofactor involved in the enzymatic reaction, or in a labeled ligand bound to the target molecule, that produces a zone of activity associated with the compounds.
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申请公布号 |
US5856083(A) |
申请公布日期 |
1999.01.05 |
申请号 |
US19950553056 |
申请日期 |
1995.11.03 |
申请人 |
PHARMACOPEIA, INC. |
发明人 |
CHELSKY, DANIEL;BURBAUM, JONATHAN J. |
分类号 |
C07B61/00;C07C205/59;C07D311/08;C07D311/22;C07D311/96;C07D491/10;C07D493/10;C07D495/10;C12Q1/00;G01N33/573;(IPC1-7):C12Q1/00 |
主分类号 |
C07B61/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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