| 摘要 |
A polypeptide consisting essentially of the NAB and linking region of an alpha -subunit of Shaker-like potassium ion channel which binds to a core region of a beta -subunit of said Shaker-like potassium ion channel. A related polypeptide is also provided and consists essentially of the core region of a beta -subunit of a Shaker-like potassium ion channel which binds to the NAB and linking region of an alpha -subunit of said Shaker-like channel. Nucleic acid sequences which encode these polypeptides, vectors containing those sequences, expression systems, hosts cells containing the aforesaid polypeptides, and pharmaceutical formulations of the peptides are also provided. Other aspects of the invention include methods of modulating the flow of potassium ions through a cell membrane surrounding a cytoplasm by introducing either of the aforesaid polypeptides or exogenous Kv beta 2 protein into the cytoplasm of the cell, methods of detecting a molecule that binds to either of the aforesaid polypeptides, and an improved yeast two-hybrid system which utilizes the aforesaid polypeptides.
|
