METHOD FOR ISOLATING AN INTRACELLULAR SUBSTANCE

摘要

The invention concerns a method for isolating a nucleic and/or protein intracellular substance, from a cell sample suspended in a liquid, comprising the following steps: exposing the sample to at least an electric pulse, whereof at least one characteristic is determined for a partial or complete lysis of the intracellular substance; separating from the lysed cell substance a nucleic and/or protein fraction. The invention is characterised in that: a) the duration of the electric pulse, defined by the time interval during which the voltage is not less than 50 % of the maximum voltage, is not less than 200 ms; and b) after separating protein fraction from the nucleic fraction of the lysed cells, the nucleic and/or protein fraction can be directly subjected to at least a treating step.