PURIFICATION OF HIGHER ORDER TRANSCRIPTION COMPLEXES FROM TRANSGENIC NON-HUMAN ANIMALS

摘要

Purification of higher order transcription complexes from transgenic non-human animals The invention relates to transgenes comprising DNA encoding for epitope-tagged TATA-box binding protein (TBP), the production of transgenic animals which express epitope-tagged TBP and use thereof for affinity purification of (novel) transcription factors and transcription complexes from a variety of eukaryotic t issues and cell-types.