发明名称 |
Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefore |
摘要 |
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
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申请公布号 |
US5834203(A) |
申请公布日期 |
1998.11.10 |
申请号 |
US19970917213 |
申请日期 |
1997.08.25 |
申请人 |
APPLIED SPECTRAL IMAGING |
发明人 |
KATZIR, NIR;WINE, DAVID;GARINI, YUVAL;CABIB, DARIO |
分类号 |
G01N21/64;G01N15/14;G01N21/78;G01N33/533;G01N33/566;G01N33/58;G02B21/06;(IPC1-7):C12Q1/68;G02B21/16;F21V9/08 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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