发明名称 |
PLASMID |
摘要 |
<p>A plasmid vector characterized by having a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of target genes and a replication origin that increases the number of copies under the induction by foreign factors; methods of expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction enzyme; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vecto r which can introduce a foreign target gene encoding proteins which are fatal or noxious to hosts into said hosts, a method of efficiently expressing the proteins by using the vector, and also a method of permitting a restriction enzyme gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult according to prior art.</p> |
申请公布号 |
EP0859057(A1) |
申请公布日期 |
1998.08.19 |
申请号 |
EP19970905474 |
申请日期 |
1997.03.10 |
申请人 |
TAKARA SHUZO CO. LTD. |
发明人 |
SAGAWA, HIROAKI;UENO, HARUMI;OSHIMA, ATSUSHI;KATO, IKUNOSHIN |
分类号 |
C12N15/09;C12N1/21;C12N9/10;C12N9/16;C12N9/22;C12N15/55;C12N15/69;C12R1/19;(IPC1-7):C12N15/67 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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