发明名称 |
Gene targeting in animal cells using isogenic DNA constructs |
摘要 |
The present invention provides novel methods for modifying the genome of an animal cell which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a "targeting DNA") that is substantially isogenic with a DNA in the cell genome (a."target DNA"); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.
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申请公布号 |
US5789215(A) |
申请公布日期 |
1998.08.04 |
申请号 |
US19970908348 |
申请日期 |
1997.08.07 |
申请人 |
GENPHARM INTERNATIONAL |
发明人 |
BERNS, ANTON;ROBANUS MAANDAG, ELS;TE RIELE, HEIN |
分类号 |
C12N15/85;C12N15/90;(IPC1-7):C12N5/06;C12N15/00;C12N15/09;C12N15/11 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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