Chaperone fragments

摘要

An E. coli GroEL polypeptide fragment (191-376) being the optical domain is coupled to a chromatographic resin (Ni-NTA Agarose). A denatured solution of cyclophilin A of known specific activity prior to denaturation is loaded onto a column of the GroEL polypeptide resin and eluted in a refolding buffer. The cyclophilin A is refolded (reconditioned) such that its specific activity is > 100 % of what it was prior to denaturation.