| 摘要 |
A device and method for growing tissue or cells on both sides of a membrane includes a cylindrical body which may be stood on either end in a culture well. A frame is slidably mounted in the body and carries a membrane, on both sides of which tissue or cells are to be grown. A tissue or cell sample is placed on the upper surface of the membrane and media is supplied to the well on the outside of the body. Passages at the bottom of the body allow the media to flow to the membrane and nurture the sample to cause its growth. When the first monolayer is grown, the body is inverted and the frame is pushed to the other end of the body, preferably by a jig supplied for that purpose. A second cell sample is deposited on the other side of the membrane, again on top of the membrane and it is nurtured by media supplied primarily from the reservoir on top and secondarily through the membrane.
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