DERIVATIVES OF 6,8-DIFLUORO-7-HYDROXYCOUMARIN

摘要

The present invention comprises 6,8-difluoro-7-hydroxycoumarins and derivatives of 6,8-difluoro-7-hydroxycoumarins, including reactive dyes, dye-conjugates and enz yme substrates. These fluorine-substituted fluorescent dyes typically possess greater photostability a nd lower pH sensitivity in the physiological pH range than their nonfluorinated analogs, exhibit less fluor escence quenching when conjugated to a substance, possess absorption and emission spectra that closely match those of their nonfluorinated analogs, and also exhibit higher quantum yields than their nonflu orinated analogs.