Concentrator for separating small samples in a centrifuge

摘要

A concentrator for concentrating a sample solution including molecules in solution which are larger than their molecular weight cutoff of a membrane in the concentrator. The concentrator has a sample reservoir which includes a bottom floor having an annular central wall surrounding a central opening. A membrane is held on a membrane support member positioned below the sample reservoir and a filtrate vial is held below the membrane to collect liquid which is passed through the membrane. A retentate vial is held over the top of the reservoir and after the sample has been concentrated the filtrate vial is removed and the remaining part of the assembly inverted. The assembly is then replaced in the centrifuge and spun to transfer the concentrated sample into the retentate vial. The membrane support member is made from spokes with open spaces between the spokes.