发明名称 |
Expression and secretion of mature human beta interleukin-1 in Bacillus subtilis and means and methods for its achievement |
摘要 |
A new plasmid vector is described which can replicate in B. subtilis and which can express and secrete, in the culture medium, human beta interleukin-1 (beta IL-1) without amino acid sequences extraneous to the natural molecule, a strain of B. subtilis transformed by the vector and a method for the expression and secretion of mature human beta interleukin-1. The human beta interleukin-1 thus obtained shows a specific acitvity of 1x109 U/mg of protein and is particularly suitable as a stimulant for the immune system, as an adjuvant in vaccines for the activation of the repair mechanism in cases of tissue damage, and for the treatment of auto-immune diseases in man.
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申请公布号 |
US5728571(A) |
申请公布日期 |
1998.03.17 |
申请号 |
US19950405775 |
申请日期 |
1995.03.17 |
申请人 |
ENIRICERCHE S.P.A. |
发明人 |
VELATI BELLINI, ADA;GALLI, GIULIANO;LUCCHESE, GIUSEPPE;GRANDI, GUIDO |
分类号 |
C07K14/545;C12N1/21;C12N15/25;C12N15/75;(IPC1-7):C12N1/21;C12N15/63;C12N15/67 |
主分类号 |
C07K14/545 |
代理机构 |
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地址 |
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