Verfahren zur Herstellung von Säugetieren mit definierten genetischen Eigenschaften

摘要

The invention pertains to a process for developing mammals with definite genetic features, including transgenic mammals. In order to obtain in one single step animals entirely derived from ES cells (embryonic stem cells), in vitro genetically engineered and cultured totipotent cells (embryonic stem cells or embryonic germ cells) are injected into tetraploid blastocysts, and the resulting embryo is implanted into a surrogate mother.