摘要 |
Gene mutations or polymorphisms are surely detected, identified or determined by simple procedures within a short period of time directly from specimens containing the gene mutations or polymorphisms in a trace amount within specific regions in target nucleic acids. A method for examining nucleic acids which comprises amplifying a specific region of a target nucleic acid in a specimen to thereby prepare a double stranded sample DNA, adding an excessive amount of the above-mentioned sample DNA to a labeled standard DNA consisting of a double stranded nucleic acid which has a site capable of binding to a solid phase carrier in one strand and a detectable label on another strand to effect competitive hybridization, detecting the above-mentioned labeled standard DNA thus reconstituted with the use of the detectable label and the site capable of binding to a solid phase carrier, and thus determining the degree of the substitution of the complementary strands between the abovementioned sample DNA and the above-mentioned labeled standard DNA to thereby detect the target DNA which is identical with the above-mentioned labeled standard DNA contained in the above-mentioned sample DNA, characterized in that the detection limit of the target DNA which is identical with the abovementioned labeled standard DNA contained in the above-mentioned sample DNA is preliminarily specified and, in the step of the competitive hybridization, the extent of the excessiveness of the above-mentioned sample DNA to be added to the above-mentioned labeled standard DNA is specified depending on the detection limit thus specified; and examination kits for detecting, identifying and determining nucleic acids carrying gene mutations or polymorphisms in accordance with this examination method.
|