Compartmentalized multiple well tissue culture plate

摘要

A cell culture device comprising a container having a plurality of wells. Each well includes a cell culture compartment defined by a lower gas permeable film and an upper sheet selectively permeable to compounds of selected sizes. The device allows culture medium to reside between the upper sheet and the lower gas permeable film. A basal medium compartment is located above the upper sheet and allows basal medium to reside upon the upper sheet. Each compartment contains an access port. A gas film support below and in partial contact with the gas permeable film holds the gas permeable film in a substantially horizontal position so that suspension or adherent cells can distribute across the surface of the gas permeable film.