Mutagenesis methods and compositions

摘要

PCT No. PCT/US92/05573 Sec. 371 Date Dec. 28, 1993 Sec. 102(e) Date Dec. 28, 1993 PCT Filed Jul. 1, 1992 PCT Pub. No. WO93/01583 PCT Pub. Date Jan. 21, 1993Methods and reagents for oligonucleotide-directed mutagenesis of a target nucleic acid are provided. In these methods a mutagenic oligonucleotide introduces a desired mutation at one site and, at a second site, introduces or eliminates a restriction site, allowing one to screen for the desired mutation by restriction analysis. Also provided are vectors and kits for performing such mutagenesis methods.