发明名称 |
CHONDROITINASES I AND II, METHODS OF PREPARATION, AND USE THEREOF |
摘要 |
Chondroitinase II, a protein having an isoelectric point of approximately 8.4-8.45 and an apparent molecular mass of 112 kDa when electrophoresed in a 4 to 20% gradient acrylamide gel in 25 mM Tris/192 mM glycine buffer at pH 8.5 in the presence of about 0.1% (w/v) SDS, has been isolated and purified. A process for the copurification by affinity chromatography of the chondroitinase I and chondroitinase II proteins produced by Proteus vulgaris is also provided. The proteins can be further purified by metal chelating chromatography. Therapeutic or surgical compositions of isolated chondroitinases I and II or the copurified mixture of chondroitinase I and II are also disclosed. These compositions are used in a method for selectively and completely disinserting the vitreous body from the neural retina of an eye. |
申请公布号 |
EP0756636(A4) |
申请公布日期 |
1997.12.17 |
申请号 |
EP19950918301 |
申请日期 |
1995.04.24 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
KHANDKE, KIRAN, MONOHAR;GOTTO, JOHN;EUL, URSULA |
分类号 |
C12N15/09;A61K35/74;A61K38/00;A61K38/46;A61K47/48;A61P27/02;C12N9/88;C12Q1/34;C12R1/01 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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