发明名称 |
ISOLATION AND CHARACTERIZATION OF ALLERGEN-BINDING CELLS FOR DIAGNOSIS OF HYPERSENSITIVITY |
摘要 |
Methods and compositions are provided for the diagnosis of allergen hypersensitivity in a patient. Rare, allergen-specific cells are enriched from a complex cell population, e.g. a patient blood sample. The percentage of blood cells that bind to a particular allergen is less than 0.01 %. The allergen-specific cell population is enriched by magnetic cell sorting. In normal blood, the allergen-binding cells are primarily B-cells expressing CD19 and CD21. In blood from allergic patients, an additional population of effector cells, e.g. basophilic granulocytes is labeled by the allergen.
|
申请公布号 |
WO9746880(A1) |
申请公布日期 |
1997.12.11 |
申请号 |
WO1997EP02951 |
申请日期 |
1997.06.06 |
申请人 |
MILTENYI BIOTEC GMBH;IRSCH, JOHANNES;MILTENYI, STEFAN;RADBRUCH, ANDREAS |
发明人 |
IRSCH, JOHANNES;MILTENYI, STEFAN;RADBRUCH, ANDREAS |
分类号 |
G01N33/543;G01N33/569;(IPC1-7):G01N33/543 |
主分类号 |
G01N33/543 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|