DNA MARKER LOCATING NEAR MALE STERILITYRESTRATION GENE IN RICE CYTOPLASM AND DNA DIAGNOSIS

摘要

PROBLEM TO BE SOLVED: To simply and exactly judge the fertility restoration plasma of rice plants by confirming whether there is the codominant DNA marker for fertility restoration gene or not through the PCR technique using the rice plant DNA as a template and a primer and by identifying the gene type. SOLUTION: This invention relates to a method for discriminating the fertility restoration plasma of rice plants in which the rice plant DNA is used as a template and the codominant DNA marker within 4.7 centimorgan near the fertility restoration gene in the rice gene is specifically amplified by the PCR technique using rice plant DNA as a template and a primer to judge whether the codominant DNA marker is present or absent. In addition, the length of its DNA fragment is detected on the difference between the case of 409 bp and the case of 425 bp whereby the fertility restoration gene are discriminated into three types. Thus, the growth of hybrid rices and their parent lines can be done with labor saved an enables the purity control of the seeds of hybrid rice by this gene plasma judgment.