LOW-TEMPERATURE ALKALI PROTEASE S, MICROORGANISM CAPABLE OF PRODUCING THE SAME, PRODUCTION OF THE SAME, AND DETERGENT COMPOSITION CONTAINING THE ENZYME AND ENZYME PREPARATION FOR FOOD PROCESSING

摘要

PROBLEM TO BE SOLVED: To obtain the subject new enzyme, capable of thoroughly hydrolyzing casein, also acting on hemoglobin or keratin, and useful for detergents, cosmetics, food modification, as a digestive auxiliary, antiinflammatory agent, etc., by culturing Serratia sp. KSM-T52. SOLUTION: This enzyme, a new low-temperature alkali protease S, is obtained by culturing Serratia sp. KSM-T52 (FERM P-15458), being useful for detergents, cosmetics, food modification, as a digestive auxiliary, antiinflammatory agent, etc. This new enzyme has the following characteristics: acting at least 0-50 deg.C; optimum temperature is 28-32 deg.C; acting pH is 5-11; optimum pH is 9-10; capable of thoroughly hydrolyzing casein; also acting on hemoglobin, keratin; estimated molecular weight determined by sodium dodecylsulfate -polyacrylamide gel electrophoresis is 51,000±1,000; and subject to inhibition by Hg<2+> , Cu<2+> , ethylenediaminetetraacetic acid, ethylene glycol bis(2-aminoethyl ether) tetraacetic acid, dithiothreitol.