摘要 |
Immunoassay for an analyte comprises: (a) incubating the analyte with an immobilised reagent, a labelled reagent and a third reagent, where the analyte, the immobilised reagent and the labelled reagent all compete for binding to the third reagent; (b) separating bound label from unbound label; (c) measuring the signal from the bound label, and (d) determining the analyte concentration by comparing the measured signal with a standard curve that has been established under the same conditions or has been theoretically calculated. |