METHOD FOR USING YEAST ADHIT PROMOTER SYSTEM FOR PRODUCING DIFFERENT KIND OF PROTEIN WITH HIGH YIELD BY BIOENGINEERING

摘要

Production of a recombinant protein (I) using a yeast strain that expresses a (I)-encoding heterologous gene under the control of an alcohol dehydrogenase (ADH) II promoter system comprises: (a) inoculating a production medium with a preculture of the yeast; (b) immediately initiating glucose supply at a constant rate of 0.7-1.4 g/l/hr; (c) maintaining the culture under aerobic conditions for about 2 days, and (d)isolating (I) from the culture broth.