发明名称 |
Plasmids for production of human growth hormone or polypeptide analog thereof, hosts containing the plasmids, products manufactured thereby, and related methods |
摘要 |
An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor CI renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator PLOL from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the vector is present in the host cell.
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申请公布号 |
US5637495(A) |
申请公布日期 |
1997.06.10 |
申请号 |
US19940340045 |
申请日期 |
1994.11.14 |
申请人 |
BIO-TECHNOLOGY GENERAL CORP. |
发明人 |
GORECKI, MARIAN;LEVANON, AVIGDOR;OPPENHEIM, AMOS;VOGEL, TIKVA |
分类号 |
A61K38/00;C07K14/61;C12N9/02;C12N15/73;(IPC1-7):C12N15/00;C12N15/70 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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