PEROXIDASE PREPARATION PROCESS WITH CULTURED SWEET POTATO CELL

摘要

Disclosed is the mass production process of the peroxidase using anion exchange resin, which is possible to separate and produce the sweet-potato peroxidase in large quantities by culturing a sweet potato culture cell strain. The process comprises the following steps of; A callus is induced by cutting apical meristem from a new shoot of the potato, and then culturing it on a solid medium containing 2,4-dichlorophenoxy acetic acid. A cell strain having a high peroxidase activity and a good cell proliferation in the induced callus is separated to obtain the mass producing cell strain by culturing it again on the same medium. The mass producing cell strain, which can produce the peroxidase, is cultured in a nutrient medium under a suitable suspension condition. An ethylene or abscisic acid are added to a culture fluid on the way of cultivation, wherein improving the peroxidase productivity remarkably.