发明名称 |
METHOD FOR DETECTING NUCLEIC ACID SEQUENCES USING COMPETITIVE AMPLIFICATION |
摘要 |
The present invention provides a method for quantitatively detecting the amount of a target nucleic acid sequence which may be present in a test sample. A test sample which may contain a target nucleic acid sequence comprising target sequences X and Y is contacted with two primer sets, the first set being specific for target X and the second set being specific for target Y. The test sample also is contacted at the same time with an internal standard sequence IS, which is substantially derived from a combination of the first and second target sequences, and its corresponding oligonucleotide primers. Haptens are associated with the oligonucleotide primer sets in such a way that amplified target sequence products X and Y are detected by capture on a solid phase to which anti-hapten capture reagents are attached. A signal ratio of (X + Y)/S is determined to quantitate the amount of the target nucleic acid sequence contained in the sample.
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申请公布号 |
CA2223823(A1) |
申请公布日期 |
1996.12.19 |
申请号 |
CA19962223823 |
申请日期 |
1996.06.03 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
MUSHAHWAR, ISA K.;BIRKENMEYER, LARRY |
分类号 |
C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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