HUMAN PROINSULIN DERIVATIVE AND PRODUCTION OF HUMAN INSULIN

摘要

A process for preparing human insulin, which comprises the steps of: preparing a DNA encoding a human proinsulin derivative having the following formula (I); inserting the DNA into a vector to construct an expression vector for the expression of the human proinsulin derivative; transforming a host cell with the expression vector; culturing the resulting transformant under a condition that allows the expression of the human proinsulin derivative; purifying human proinsulin derivative from the culture; and preparing human insulin from the human proinsulin derivative by an enzymatic hydrolysis:B chain-Z-A chain(I)Wherein, said A and B chains are respectively human insulin chains; Z is a peptide of formula U-U-X-P-J-(X')n-U-U; U is an arginine or lysin residue; X and X' are independently any amino acid residue; P is a proline residue; J is a glycine, arginine or lysine residue; and n is 0 or an integer of 1 to 5.