| 摘要 |
<p>The present invention provides for the parallel detection of polynucleotide species size separated according to multiplex sequencing protocols, which are advantageously substituted for previous serial reprobing methods. This parallelism is realized by separately analyzing species handled by multiplex strategies in a length stratified manner. Stratification is accombplished by recovering size fractionated or size separated fractions, and examining these separately. The effort of analyzing many fractions is far offset by the advantages realized by parallel tag detection or probing; while a few hundred fractions may correspond to nested sets of fragments of up to tens or hundreds of bases in length, each fraction yields information about thousands to millions of contiguous sequences. Parallel tag detection is faborably accomplished by hybridization of multiplex sample with one or more stationary arrays of tag specific or sequence-segment specific probes. Information thus obtained is reassembled, according to the presence or absence of a tag, or base-dependent label associated with a tag, in each such fraction and the order of each such fraction in a contiguous series, generally by algorithmic means, to yield linear sequence information.</p> |
