发明名称 |
Analytical methods using chemiluminescent dioxetane derivatives |
摘要 |
Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light. The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. <IMAGE> |
申请公布号 |
IL118935(D0) |
申请公布日期 |
1996.10.31 |
申请号 |
IL19960118935 |
申请日期 |
1996.07.24 |
申请人 |
THE BOARD OF GOVERNORS OF WAYNE STATE;BAYER CORPORATION |
发明人 |
ADOLFSEN ROBERT H;AKHAVAN-TAFTI HASHEM;DE SILVA RENUKA;SCHAAP PAUL A |
分类号 |
G01N21/78;C07D321/00;C07F9/54;C09K11/06;C09K11/07;C12Q1/42;G01N33/53;G01N33/58;(IPC1-7):G01N |
主分类号 |
G01N21/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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