| 摘要 |
PURPOSE: To readily and efficiently produce trehalose useful for medicines, food preservatives, low-caloric sweeteners, etc., by reacting a microorganism, etc., belonging to the genus Pseudomonas and having the ability to convert maltose into trehalose. CONSTITUTION: A microbial cell or its treated substance of a microorganism, belonging to the genus Pseudomonas and having the ability to convert maltose into trehalose [e.g. Pseudomonas sp. F-1 (FERM P-14747)] is added to a 0.1M phosphoric acid buffer solution (pH 7.0) containing maltose in the presence of a suppressant suppressing interfering enzym contaminant (e.g. ethylenediaminetetraacetic acid), reacted at 37 deg.C for 24hr and then centrifuged. The resultant supernatant liquid is treated by preparative high-performance liquid chromatography to separate and purify the product. Thereby, the objective trehalose useful as a medicine, a diagnostic agent, an enzyme or a preservative for foods, a water holding agent for cosmetics, a low-calorie sweetener, etc., is readily and efficiently be obtained. |
