AFFINITY CHROMATOGRAPHIC METHOD FOR SCREENING COMBINATORIAL LIBRARIES

摘要

An assay method for determining the affinity of low molecular weight ligands for a biological of interest under physiologically relevant conditions, which method comprises immobilising the biological of interest on a solid phase matrix, contacting this with a mixture comprising the ligands, removing any unbound ligands from the matrix, eluting the ligands from the matrix and establishing their affinity for the biological of interest by reference to their order of retention on the column.