| 摘要 |
Novel cancer cell inhibitors have been isolated from the venoms of poisonous snakes Crotalus atrox and Naja n. kaouthia; they are called Atroporin and Kaotree, respectively. The cancer cell inhibitors Atroporin and Kaotree are characterized as potent anti cancer agents. Atroporin and Kaotree, individually, or combined together selectively kill various types of cancer cells in concentrations as low as 0.5 mu g/ml. Atroporin and Kaotree are characterized as stable protein components of venoms consisting of peptides whose molecular weights are approximately 35,000 and 6,000 daltons, revealed by gel electrophoresis. The partial sequence of Atroporin for its first fifteen N-terminal amino acids is:SEQ ID: 1 =[Val, Ile]-[Val, Ile]-Gly-Gly-Asp-Glu-(Cys)-Asn-Ile-Asn- Glu-His-Arg-Ser-Leuand, similarly for Kaotree:SEQ ID: 2=Met-Glu-Cys-Tyr-Arg-Met-Ser-Asn-Ile-Val- Thr-Cys-Gln-Pro-Trp.Neither of these sequences appear in standard data bases including all known proteins, therefore they are novel proteins. Atroporin and Kaotree have cytolytic, i.e. killing effect, on various types of cancer cells and are claimed as versatile anti cancer agents. Furthermore, Atroporin and Kaotree are claimed as therapeutic agents for a wide range of cancers such as: breast, colon, liver, lung pharynx ovary etc. It is also an object of this invention to provide two different embodiments such as Atroporin and Kaotree, or the combination of both, as compositions for the treatment of various types of cancers as a biotherapy.
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