| 摘要 |
<p>The present invention provides a method of modulating gene expression by co-infection of cells with two or more interdependent replication-deficient adenoviral vectors, in particular by introducing into a cell, in any order or simultaneously, a first replication-deficient adenoviral vector comprising a first promoter and a first nucleic acid sequence which encodes a first gene product, wherein the first promoter regulates expression of the first gene product, and (b) a second replication-deficient adenoviral vector comprising a second promoter and a second nucleic acid sequence which encodes a second gene product, wherein the second promoter regulates in cis expression of the second gene product and is regulated in trans by the first gene product, and wherein the first promoter is different from the second promoter. The present invention also provides a method of modulating gene expression to produce a gene product, e.g., protein, a method for increasing the selective targeting for gene delivery of a particular type of cells, and interdependent adenoviral vectors appropriate for use in the methods of the present invention.</p> |
