| 摘要 |
<p>Two groups of hybrid suicide genes are disclosed, where the genes of one group specifically activate 5-fluorocytosine, a pyrimidine nucleobase analogue, while the genes of the other group activate azidothymidine, a pyrimidine nucleobase analogue, into derivatives toxic for mammalian cells. A variety of methods are also disclosed for selectively killing transfected tumour cells or immune cells using a single hybrid suicide gene or a combination of two hybrid suicide genes selected for the complementarity of their antimetabolic activities. Furthermore, eukaryotic vectors including two suicide gene expression units, i.e. a first unit sensitising the tumour cells to 5-fluorocytosine or 5-fluorouracil, and a second making HIV-infected cells synergistically resistant to azidothymidine.</p> |
