发明名称 |
METHOD OF PREPARING OLIGONUCLEOTIDE PROBES OR PRIMERS, VECTOR THEREFOR AND USE THEREOF |
摘要 |
A method of preparing a nucleic acid sequence capable of hybridizing to a target DNA sequence without requiring knowledge of this DNA sequence comprises the steps of ligating or otherwise linking a DNA fragment related to the target DNA sequence to a nucleotide sequence containing the recognition motif of an asymmetrically cleaving restriction enzyme, and subjecting the construct to the restriction enzyme to thereby cleave the construct within the target-related DNA fragment part thereof. A vector therefor comprises a site for insertion of the target-related DNA fragment, and a recognition sequence for an asymmetrically cleaving restriction enzyme or enzymes on one or both sides of said insertion site.
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申请公布号 |
WO9614406(A1) |
申请公布日期 |
1996.05.17 |
申请号 |
WO1995SE01319 |
申请日期 |
1995.11.07 |
申请人 |
LANDEGREN, ULF;SUNDVALL, MATS |
发明人 |
LANDEGREN, ULF;SUNDVALL, MATS |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12N15/10;C12N15/66 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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