| 摘要 |
A method of determining tubulin protein polymerization activity in a test sample is disclosed. The method comprises: combining the test sample with a polymerizable tubulin protein; contacting said test sample and polymerizable tubulin protein with a material which traps polymerized tubulin protein; measuring the amount of polymerised tubulin protein trapped by said material, and comparing the amount of polymerized tubulin protein trapped by said material to a standard. A method of determining tubulin protein polymerization inhibition in a test sample is also disclosed, which comprises: combining said test sample with a polymerizable tubulin protein and a pre-determined amount of a compound which causes tubulin protein polymerization to form a mixture; contacting said mixture with a material which traps polymerized tubulin protein; measuring the amount of polymerized tubulin protein trapped by said material, and comparing the amount of polymerized tubulin protein trapped by said material to a standard. |
