| 摘要 |
PURPOSE: To obtain a type C hepatitis virus gene having a specific base sequence, coding for a type C hepatitis virus protein, expressing the protein in a host by integrating into an expression vector and producing a protein useful for diagnosis, treatment, etc., of type C hepatitis infecting through blood transfusion, etc., CONSTITUTION: This is a new type C hepatitis virus gene having a base sequence containing the base sequence of the formula, containing an RNA coding for a type C hepatitis virus protein and capable of producing a type C hepatitis virus protein useful as a diagnostic of type C hepatitis by the detection or classification of a type C hepatitis virus by means of antigen-antibody reaction, a vaccine for treating type C hepatitis, etc., by gene technology. The gene is prepared as follows: a type C hepatitis virus genome RNA is extracted from a serum of a type C hepatitis patient; a cDNA is synthesized with a reverse transcriptase using the genome RNA as a template; the cDNA is digested with an appropriate restriction enzyme, the obtained fragments are transduced into a plasmid, and the gene is integrated into a host cell; and the obtained recombinant DNAs are cloned and the objective gene is recovered from a positive clone. |
