PROCESS FOR PRODUCING URINARY TRYPSIN INHIBITOR AND DOMAINS THEREOF, NOVEL POLYPEPTIDE RELATED TO THE BOTH, AND PROCESS FOR PRODUCING THE POLYPEPTIDE

摘要

A process for producing a urinary human trypsin inhibitor (UTI) and domains thereof by using a yeast of the genus Pichia as the host cell; an expression system related thereto; a novel polypeptide at least having the amino acid sequence represented by formula (I) in the specification; a DNA coding for the same; an expression vector containing the DNA; a transformant containing the vector; and a process for producing the polypeptide. The above UTI, expression system of each domain thereof, and process for producing the UTI and domains thereof enable UTI and domains thereof to be mass-produced readily and efficiently. The use of the expression system facilitates the preparation of variants or derivatives of UTI and so forth and contributes to the development of novel medicines such as a protease inhibitor. The invention also provides a novel useful polypeptide which is more excellent in elastase inhibitory activity than natural UTI and can be mass-produced.