Amplification of midivariant DNA templates

摘要

Inter alia, an amplification method for detecting the presence of a target nucleotide acid sequence in a test sample characterized in that it comprises: hybridizing at least two template sequence/probe conjugates to a target nucleic acid sequence, each conjugate including a distinct non-replicable portion of a template sequence and a distinct target specific nucleic acid sequence complementary to a target nucleic acid sequence in a test sample, where the hybridization of at least two target specific nucleic acid sequences causes them to be contiguously aligned on the target sequence; ligating the conjugates to form a replicable template; and replicating the template.