| 摘要 |
The present invention relates to iminium ion substitute cyanine dyes having a fluorescence absorbance of between about 500 and 900 nm, a reduced tendency to aggregate and enhanced photostability. The cyanine dyes of the present invention are represented by formula (I) wherein n is 0, 1, 2 or 3; m is 0, 1, 2 or 3; R1 and R2 are taken together to form an aromatic ring or a fused polycyclic aromatic ring; R3 and R4 are taken together to form an aromatic ring or a fused polycyclic aromatic ring; R5 and R6 are independently selected from the group consisting of (CH2)pX where p is 1-18 and X is a functional group that reacts with amino, hydroxy and sulfhydryl nucleophiles; R7 and R8 are independently selected from the group consisting of hydrogen; C1-C10 alkyl groups and where R7 and R8 are taken together to form a five- or six-membered heterocyclic ring; R9 are each independently selected from the group consisting of hydrogen, alkyl and where more than one R9 are taken together to form a five- or six-membered ring; Y is selected from the group consisting of C(CH3)2, S, O and Se; and Z is selected from the group consisting of C(CH3)2, S, O and Se. The present invention also relates to a method for using the cyanine dyes of the present invention to fluorescent label molecules, particularly biomolecules such as antibodies, DNA, carbohydrates and cells.
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