摘要 |
A process for determining the reticulocyte population in blood samples, which process includes the use of coriphosphine O to stain reticulocytes and which process is particularly suitable for detection by flow cytometry techniques. The optics (10) of a flow cytometer useful in the process includes an argon ion laser (12) as a light source which illuminates a blood sample flowing through a flow cell (14). Scattered light and fluorescence fall upon a dichroic mirror (22) which serves to reflect the right-angle scattered light (24) and transmit the fluorescence (26). A dichroic mirror (30) serves to reflect green fluorescence (32) and transmit red fluorescence (38). |