| 摘要 |
A method for modulating the activity of a protein or nucleic acid in vivo by binding it with an oligonucleotide wherein at least one nucleotide unit includes an aminohydrocarbon phosphonate moiety having formula (I), wherein X is (II), and wherein R1 is a hydrocarbon, preferably alkylene, and R2, R3 and R4 are each independently hydrogen or a hydrocarbon. Preferably, R1 is methyl, ethyl or propyl moiety. It is also preferred to use the pure stereoisomers of such compounds rather than their racemic mixtures. Such pure stereoisomers having improved binding capabilities and improved resistance to nucleases. Alternatively, X may be (III), wherein R1, R2, and R3 are as hereinabove described, and R5 is a detectable marker, thus making such oligonucleotides useful as diagnostic probes or for screening libraries of cells.
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